Ticari Meyve Sularında HPLC ile Şeker Analizi için Standart Bir Metot Teyidi

This study aimed to verify the standard test method TS EN 12630 and determine sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should parameters in order show its performance for analysis, under consideration. For this purpose, Sucrose, Glucose Fructose were analysed orange obtained from market. The principle is based on separation sugars a cation-exchange resin isocratic elution with mobile phase, detection using differential refractive index (RI) detector external method. Accuracy precision performed via intraday inter day studies accuracy (generally accepted as repeatability reproducibility) recovery values added into juice sample 92%, 99% 96% sucrose, glucose fructose, respectively.